Cloned HEK Parental ChemiBrite with Gαqs cells were made by stable transfection of HEK293 cells with optimized quantities of plasmid encoding a novel variant of clytin and human Gαqs chimera. This photoprotein stability-tested cell line is ideal for recombinant expression in luminescent analysis of agonists, antagonists and modulators at the target protein, as well as cAMP assays.