Ready-to-Assay™ FFA1/GPR40 Free Fatty Acid Receptor Frozen Cells

FFA1/GPR40 is a Gi/Gq-coupled GPCR that is activated by free medium and long-chain fatty acids. FFA1 receptors are expressed in brain tissue and insulin-producing β Pancreatic islets (Briscoe et al., 2003). FFA1 receptors regulate insulin release from islets in response to long-chain fatty acids in the plasma (reviewed in Brown et al., 2005). Cloned human FFA1 receptor-expressing ChemiBrite cells were made by stable transfection of HEK293 cells with ChemiBrite clytin and the FFA1 receptor. The cells have been cryopreserved at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent, fluorescent and cAMP accumulation analysis of agonists, antagonists and modulators at the FFA1 receptor.

Catalog No. Size Price Quantity
HTS038RTA 2 Vials $769.00

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Application
Calcium Flux Assay
Family
Free Fatty Acid
Target
FFA1/GPR40
Accession Number
NM_005303
Species
Human
Host Cell Type
Chem-1
Content
2 Vials (1 ml/vial) of mycoplasma-free cells for one 96-well plate. Includes cell plating media (50 mL).
Storage
Cell vials are to be stored in the vapor phase of liquid N2. Media Component at 4ºC (or -20ºC for prolonged storage).