Ready-to-Assay™ PAR2 Receptor Frozen Cells

The protease-activated receptor family of GPCRs has a unique mechanism of activation, in which protease cleaves a prodomain to reveal a peptide sequence that functions as a tethered ligand to activate the receptor (Macfarlane et al., 2001). PAR2 is specifically activated by trypsin and mast cell tryptase, and can also be activated by free peptide analogs of the tethered ligand, such as SLIGRL and furoyl-LIGRLO (Coelho et al., 2003; Kawabata et al., 2004). PAR2 is expressed in endothelium, gastrointestinal epithelium, macrophages, eosinophils and nociceptive afferent neurons. Activation of PAR2 in these cells and tissues promotes vasodilation, inflammation, allergy, hyperalgesia and intestinal permeability. Therefore, PAR2 is regarded as an attractive therapeutic target for colitis, asthma, myocardial ischemia/reperfusion injury, and pain (Cocks et al., 1999; Hansen et al., 2005; Lindner et al., 2000; McLean et al., 2002; Vergnolle et al., 2001). Cloned human PAR2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PAR2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PAR2 and its ligands

Catalog No. Size Price Quantity
HTS037RTA 2 Vials $778.00

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Application
Calcium Flux Assay
Family
Proteinase-Activated
Target
PAR2
Species
Human
Host Cell Type
Chem-1
Content
2 Vials (1 ml/vial) of mycoplasma-free cells for one 96-well plate. Includes cell plating media (50 mL).
Storage
Cell vials are to be stored in the vapor phase of liquid N2. Media Component at 4ºC (or -20ºC for prolonged storage).