Ready-to-Assay™ GPR14 Urotensin II Receptor Frozen Cells

This product has been discontinued. Please refer to the alternate product, PathHunter® eXpress UTR2 CHO-K1 β-Arrestin GPCR Assay (93-0283E2) or PathHunter® eXpress β-Catenin Nuclear Translocation Assay (93-0743E3).

Ready-to-Assay™ GPCR frozen cells are designed for simple, rapid assays with no requirement for intensive cell culturing. Eurofins DiscoverX Products LLC. has optimized the freezing conditions to provide cells with high viability and functionality post-thaw.  Simply thaw the cells and resuspend them in media, dispense cell suspension into assay plates and, following overnight recovery follow the protocol to run the assay. Urotensin II, a cyclic 11-13 residue peptide expressed in motoneurons of the spinal cord, acts as a potent vasoconstrictor (Coulouarn et al., 1998; Ames et al., 1999). The effects of urotensin II are mediated by binding to a GPCR, GPR14, which is expressed in endothelium, smooth muscle, heart and pancreas (Ames et al., 1999). Genetic deletion of GPR14 in mice renders aortae refractile to the contractile activity of urotensin II without changing baseline hemodynamics (Behm et al., 2003). Pharmacological inhibition of urotensin II/GPR14 interactions prevents renal insufficiency following renal artery ligation (Clozel et al., 2004). Thus, GPR14 is an attractive target for a number of cardiovascular diseases. Cloned human GPR14-expressing cell line is made in the Chem-2 suspension cell line host, which supports high levels of recombinant GPR14 expression on the cell surface for functional detection via the calcium signaling pathway.

Catalog No. Size Price Quantity
HTS033RTA 2 Vials

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Application
Calcium Flux Assay
Family
Urotensin
Target
UTR2
Species
Human
Host Cell Type
Chem-2
Content
2 Vials (1 ml/vial) of mycoplasma-free cells for one 96-well plate. Includes cell plating media (50 mL).
Storage
Cell vials are to be stored in the vapor phase of liquid N2. Media Component at 4ºC (or -20ºC for prolonged storage).