Find a Product

PathHunter® U2OS VISTA/VISTA Dimerization Cell Line

The PathHunter® Dimerization assay detects ligand induced dimerization of two subunits of a receptor-dimer pair. The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA). Cytoplasmic tail may have been deleted from one or both receptor. Binding of an agonist to one receptor subunit induces it to interact with its dimer partner, forcing complementation of the two enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.


Catalog No. Size Price Quantity
93-1079C3 2 vials $19995.00
Add to Cart

Related Family Members:

Target/Description
Platelet receptor Gi24
Receptor Family
T cell receptor (inhibitory)
Coupling
N/A
Accession Number
NM_022153.1
Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Available as a Service?
No
Agonist/Ligand
Human VISTA Antibody
  • [User Manual] PathHunter® Dimerization Assay

    PathHunter® Dimerization Assays provide a robust, highly sensitive and easy-to-use cell-based functional assay to study various protein activities in a cell. The assays allow for the monitoring of receptor-receptor interactions at the&...

    [User Manual] PathHunter® Dimerization Assay

Required Products

Cat. No. Description Size Price Quantity
93-0247 PathHunter® Flash Detection Kit 125 mL 1,000/4,000 dp (96-well/384-well) $3313.00
Add to Cart
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
Add to Cart
93-0563R5A AssayComplete™ Cell Plating 5 Reagent 100 mL $299.00
Add to Cart


Recommended Products

Cat. No. Description Size Price Quantity
92-1311 Anti-VISTA Antibody [730804] 25 µg $250.00
Add to Cart
92-0013 384-well Clear bottom TC Treated, Sterile WCB, FB w/ LID 10 plates/pk $179.00
Add to Cart
92-0015 384-well White bottom TC Treated, Sterile w/ LID 10 plates/pk $179.00
Add to Cart