PathHunter® U2OS TORC2 Nuclear Translocation Cell Line

PathHunter® Nuclear Translocation cell lines are engineered to co-express two fusion proteins: a) Enzyme Donor (ED) tagged target protein; b) an Enzyme Acceptor (EA) tagged TAZ domain, derived from the CBP/P300 transcription factor, that localizes to the nucleus. Depending on the assay, activation of the signaling pathway can either a) induce translocation of the ED-tagged target protein into the nucleus, which will force complementation of the two enzyme fragments, and result in the formation of a functional enzyme that will hydrolyze substrate and generate a chemiluminescent signal; or b) induce the ED-tagged protein to vacate the nucleus, resulting in a decrease of functional enzyme and a subsequent decrease of chemiluminescent signal. Some nuclear translocation assays will also co-express an untagged secondary protein involved in the pathway of interest.

Catalog No. Size Price Quantity
93-0813C3 2 vials $20595.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.
Receptor Family
Transcription factor
Coupling
N/A
Accession Number
NM_181715
Species
Human
Prolink™ Tag
PK
Cell Background
U2OS
Agonist/Ligand
Forskolin
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control compound, using the assay conditions described below. Following stimulation, signal was detected using the PathHunter Detection Kit according to the recommended protocol. Please refer to page 2 for recommended assay reagents, detection reagents, and control compounds.
  • [User Manual] PathHunter® Pathway Assay

    PathHunter® Pathway Assays, when used in conjunction with the respective detection kit (indicated in the target specific data sheet) provide a cell-based functional assay to study various protein activities in a cell. The assays described in this booklet have been validated for use in 384-well microplate formats.

    [User Manual] PathHunter® Pathway Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2594.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $288.00
93-0563R19A AssayComplete™ Cell Plating 19 Reagent 100 mL $288.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $236.00
92-0029 AssayComplete™ Hygromycin B 20 mL $175.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $92.00
92-0028 AssayComplete™ Puromycin 1 mL $72.00