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PathHunter® U2OS TIM3/TIM3 Dimerization Cell Line

The PathHunter TIM3/TIM3 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand induced dimerization of TIM3/TIM3 receptors. This product uses EFC technology. The cell line included overexpresses TIM3 target protein fused to PK alongside TIM3 target protein fused to EA. Upon ligand-induced dimerization of TIM3/TIM3 , detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells.

Catalog No. Size Price Quantity
93-1082C3 2 vials $20599.00
For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Receptor Family
T cell receptor (inhibitory)
Coupling
N/A
Accession Number
NM_032782.4
Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
Anti-TIM3 Antibody [344801]
Cells were seeded in a 384-well plate, incubated at 37°C / 5% CO2 followed by stimulation with a control ligand, as defined in the assay conditions below. After stimulation, assay signal was detected using the PathHunter® detection kit according to the recommended protocol. Please refer to page 2 for recommended assay reagents, detection reagents, and control compounds.

  • [User Manual] PathHunter® Dimerization Assay

    PathHunter® Dimerization Assays provide a robust, highly sensitive and easy-to-use cell-based functional assay to study various protein activities in a cell. The assays allow for the monitoring of receptor-receptor interactions at the&...

    [User Manual] PathHunter® Dimerization Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0247 PathHunter® Flash Detection Kit 125 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $3449.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $349.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R5A AssayComplete™ Cell Plating 5 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $239.00
92-0029 AssayComplete™ Hygromycin B 20 mL $179.00
92-0023M AssayComplete™ Protein Dilution Buffer 2 x 50 mL $139.00
92-0030 AssayComplete™ G418 20 mL $139.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00