PathHunter® U2OS SSTR5 β-Arrestin Cell Line

PathHunter® β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0402C3 2 vials $20595.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Receptor Family
Somatostatin
Coupling
Gi/Go
Accession Number
NM_001053
Species
Human
β-Arrestin isoform
β-Arrestin-2
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
Somatostatin 28
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] PathHunter® β-Arrestin Assay for GPCR Cell Lines

    PathHunter β-Arrestin cell lines are stable clonal cell lines that expedite drug discovery and development by providing robust response to over 90% of all known G-protein coupled receptor (GPCRs), with accurate pharmacology. This assay measures an essential pathway in GPCR activation, i.e. β-arrestin recruitment to activated GPCRs, enabling scientists...

    [User Manual] PathHunter® β-Arrestin Assay for GPCR Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2594.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $288.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $288.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $236.00
92-0029 AssayComplete™ Hygromycin B 20 mL $175.00
92-0030 AssayComplete™ G418 20 mL $133.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $92.00