PathHunter® U2OS SHC1-EA Parental Cell Line

PathHunter SH2 Recruitment EA Parental Cell Lines express EA fused to the SH2 domains of adaptor proteins, like SHC1 or PLCG1. These cell lines can be used to build your own receptor tyrosine kinase (RTK), cytokine receptor kinase, or cytosolic tyrosine kinase (CTK) functional assay cell lines to monitor activation of these receptors or tyrosine kinases of interest in your research. To develop a functional assay cell line, a researcher will introduce a PK-tagged receptor, and, in some cases, an untagged co-receptor, into one of these two parental cell lines. Activation of the receptor-PK induces receptor tyrosine phosphorylation, leading to SH2-EA recruitment, and forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-1123C3 2 vials $32,479.00

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Cell Background
  • [User Manual] PathHunter® EA Parental Cell Lines

    The PathHunter EA Parental Cell Lines are engineered cell lines that stably express a particular reporter protein fused to a large β–galactosidase (β-gal) enzyme reporter fragment called EA (enzyme acceptor). The EA parental cell lines have various cell backgrounds and are intended to be used with ProLabel®/ ProLink™-tagged proteins to...

    [User Manual] PathHunter® EA Parental Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,857.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $379.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $329.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $249.00
92-0029 AssayComplete™ Hygromycin B 20 mL $227.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $109.00