PathHunter® U2OS PRβ Nuclear Translocation Cell Line

The PathHunter® Nuclear Translocation assay format detects translocation of target NHR into the nucleus using Enzyme Fragment Complementation. The cells have been engineered to express two complementary fragments of β-galactosidase that are localized to different cellular compartments: the smaller ProLabel (PL) fragment is fused to the NHR, and the larger enzyme acceptor (EA) fragment is localized in the nucleus. Activation of the NHR induces receptor translocation to the nucleus, forcing complementation of the PL and EA fragments. Activity of the resulting fully formed β-galactosidase enzyme is detected using a chemiluminescent substrate.

Catalog No. Size Price Quantity
93-0484C3 2 vials $10,299.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Receptor Family
NHR
Coupling
N/A
Accession Number
NM_000926
Species
Human
Prolink™ Tag
PL
Cell Background
U2OS
Agonist/Ligand
D(-)-Norgestrel
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] PathHunter® NHR Assay

    Nuclear Hormone Receptor (NHR) activation is comprised of four steps: 1) ligand binding to the NHR, 2) nuclear translocation, 3) recruitment of a Steroid Receptor Coactivator Peptide (SRCP), and 4) transcriptional activation of target DNA sequence. P...

    [User Manual] PathHunter® NHR Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,619.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $349.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R14A AssayComplete™ Cell Plating 14 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $239.00
92-0029 AssayComplete™ Hygromycin B 20 mL $179.00
92-0030 AssayComplete™ G418 20 mL $139.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00