PathHunter® U2OS NIK Signaling Cell Line

The PathHunter NIK Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. CD40, TWEAK) induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein. Upon activation of NFκB signaling by a ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA. This stable modified cell line is provided as two vials of cryopreserved cells.

Catalog No. Size Price Quantity
93-1059C3 2 vials $38,149.00

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Related Family Members:

Receptor Family
Ser/Thr Kinase
Coupling
N/A
Accession Number
NM_003954.3
Receptor Species
Human
Prolink™ Tag
ePL
Cell Background
U2OS
Agonist/Ligand
Recombinant Human TWEAK
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control compound. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] PathHunter® Pathway Assay

    PathHunter® Pathway Assays, when used in conjunction with the respective detection kit (indicated in the target specific data sheet) provide a cell-based functional assay to study various protein activities in a cell. The assays described in this booklet have been validated for use in 384-well microplate formats.

    [User Manual] PathHunter® Pathway Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0812 PathHunter® PL/PK Detection kit 4,000 dp (384-well) $4,549.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $389.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $309.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $309.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $249.00
92-0030 AssayComplete™ G418 20 mL $199.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00