Find a Product

PathHunter® U2OS NIK Signaling Assay

PathHunter® Degradation cell lines are engineered to express an Enzyme Donor (ED) tagged target protein. Activation of the pathway results in degradation of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-1059C3 Clone $35597.00

Related Family Members:

Receptor Family
Ser/Thr Kinase
Coupling
N/A
Accession Number
NM_003954.3
Species
Human
Prolink™ Tag
ePL
Cell Background
U2OS
Agonist/Ligand
TWEAK
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control compound. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] cAMP Hunter™ Exenatide Bioassay Kit

    cAMP Hunter Exenatide Bioassay Kits provide a functional, robust, highly sensitive, and easy-to-use cell-based assay to study Exenatide potency and neutralizing antibodies. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The pre-qualifie...

    [User Manual] cAMP Hunter™ Exenatide Bioassay Kit

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0812 PathHunter® PL/PK Detection kit 4,000 dp (384-well) $4140.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00


Select Recommended Products

Cat. No. Description Size Price Quantity
92-1297 Recombinant Human TWEAK 25 µg $556.00
92-0013 384-well Clear bottom TC Treated, Sterile WCB, FB w/ LID 10 plates/pk $179.00
92-0015 384-well White bottom TC Treated, Sterile w/ LID 10 plates/pk $179.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $89.00
92-0011 Green V-Bottom PP Ligand Dilution Plates 10 plates/pk $69.00