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PathHunter® U2OS NIK Signaling Assay

PathHunter® Degradation cell lines are engineered to express an Enzyme Donor (ED) tagged target protein. Activation of the pathway results in degradation of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer, lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.


Catalog No. Size Price Quantity
93-1059C3 2 vials $35597.00
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Related Family Members:

Target/Description
Mitogen-activated protein kinase kinase kinase 14
Receptor Family
Ser/Thr Kinase
Coupling
N/A
Accession Number
NM_003954.3
Species
Human
Prolink™ Tag
ePL
Cell Background
U2OS
Available as a Service?
Yes
Agonist/Ligand
TWEAK
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control compound. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] PathHunter® Pathway Assay

    PathHunter® Pathway Assays, when used in conjunction with the respective detection kit (indicated in the target specific data sheet) provide a cell-based functional assay to study various protein activities in a cell. The assays described in this booklet have been validated for use in 384-well microplate formats.

    [User Manual] PathHunter® Pathway Assay

Required Products

Cat. No. Description Size Price Quantity
93-0812 PathHunter® PL/PK Detection Kit 4,000 dp (384-well) $4140.00
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92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
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93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00
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