PathHunter® U2OS NIK Signaling Assay
Degradation cell lines are engineered to express an Enzyme Donor (ED) tagged target protein. Activation of
the pathway results in degradation of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer,
lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a
functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.
- Mitogen-activated protein kinase kinase kinase 14
- Receptor Family
- Ser/Thr Kinase
- Accession Number
- Prolink™ Tag
- Cell Background
- Available as a Service?
PathHunter® Pathway Assays, when used in conjunction with the respective detection kit (indicated in the target specific data sheet) provide a cell-based functional assay to study various protein activities in a cell. The assays described in this booklet have been validated for use in 384-well microplate formats.