PathHunter® U2OS mPD-L1 Ligand Cell Line

Checkpoint Ligand Cell Lines are companion products to the Checkpoint Signaling Assays, which have been engineered to exogenously express a cell surface ligand for a specific checkpoint receptor. When co-cultured with its cognate receptor-expressing cells, the ligand expressed on the Ligand Cell Line will engage with the checkpoint receptor on the Checkpoint Signaling Cell Line. This receptor-ligand engagement leads to receptor activation and downstream signaling events, which are detected by the forcing complementation of the two β-galactosidase enzyme fragments: EA-tagged SH2 domain and PK-tagged checkpoint receptor. The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-1159C3 2 vials $10249.00

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Receptor Family
IgSF (immune inhibitory receptor ligand)
Accession Number
Prolink™ Tag
Cell Background
Anti-mPD-L1 Antibody
Anti-mPD-L1 Antibody
Signaling assay cells were seeded in a 96-well plate and then pre-incubated with a control antibody, followed by coincubation with the ligand cell line using the assay conditions described below. Following stimulation, signal was detected using the PathHunter Detection Kit according to the recommended protocol. Please refer to page 2 for recommended assay reagents, detection reagents, and control compounds.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2619.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $349.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $239.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00
92-0028 AssayComplete™ Puromycin 1 mL $79.00