PathHunter® U2OS MEM-EA Parental Cell Line

PathHunter translocation EA parental cell lines take advantage of DiscoverX’s proprietary enzyme fragment complementation technology. The cell lines are engineered to express a large fragment of β-galactosidase (β-gal) called enzyme acceptor (EA) fused to a reporter protein (Reporter-EA) which is localized to a specific subcellular compartment. Introduction of a smaller fragment of β-gal called ProLink™ (PK) fused to a target protein (Target-PK) into the cells allows for development of assays to monitor translocation of the Target-PK into this subcellular compartment. Assays using these cell lines can be used to screen and study ligands (e.g. agonists/antagonists, pharmacological chaperones, etc.) or potentially other experimental manipulations that results in translocation of the target protein to the compartment where the Reporter-EA resides. Co-localization of these two proteins forces complementation of the two β-gal enzyme fragments (EA and PK), which results in a functional enzyme which hydrolyzes PathHunter detection reagents to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-1101C3 2 vials $32,479.00

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  • [User Manual] PathHunter® EA Parental Cell Lines

    The PathHunter EA Parental Cell Lines are engineered cell lines that stably express a particular reporter protein fused to a large β–galactosidase (β-gal) enzyme reporter fragment called EA (enzyme acceptor). The EA parental cell lines have various cell backgrounds and are intended to be used with ProLabel®/ ProLink™-tagged proteins to...

    [User Manual] PathHunter® EA Parental Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,857.00
93-0167 pCMV-ProLink™ 1 Vector 1 Vector $684.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $379.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $329.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $249.00
92-0029 AssayComplete™ Hygromycin B 20 mL $227.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $109.00