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PathHunter® U2OS INSRb Internalization Assay

PathHunter® RTK Internalization Cell Lines provide a quantitative measurement of total endocytosed Receptor Tyrosine Kinase (RTK) protein using β-galactosidase (β-gal) Enzyme Fragment Complementation (EFC). In this system, cells are engineered to co-express the ProLink™ (PK)-tagged RTK and the Enzyme Acceptor (EA)-tagged endosome fusion proteins. Activation of the RTK results in internalization of the receptor and trafficking to cellular endosomes, where the two enzyme fragments complement and form a functional β-gal enzyme that is capable of hydrolyzing substrate and generating a chemiluminescent singal using the PathHunter® Detection kit.

Catalog No. Size Price Quantity
93-0808C3 2 vials $20595.00

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Related Family Members:

Receptor Family
Receptor Tyrosine Kinase
Accession Number
Prolink™ Tag
Cell Background
Insulin, Human
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] PathHunter® Receptor Tyrosine Kinase Functional Assay

    PathHunter Receptor Tyrosine Kinase (RTK) Functional Assays are functional cell-based assays used for identification, screening or profiling of agonists, small molecule inhibitors and therapeutic biologics (peptides, proteins and various types of antibodies). Additionally, they have non-R&D applications as bioassays for QC testing and testing for anti-drug ...

    [User Manual] PathHunter® Receptor Tyrosine Kinase Functional Assay
  • [User Manual] PathHunter® Receptor Tyrosine Kinase Internalization Assays

    PathHunter® Receptor Tyrosine Kinase (RTK) Internalization Assays are nonimaging, non-antibody based chemiluminescent detection assays that provide a direct and quantitative measurement of internalized RTKs localized to early 

    [User Manual] PathHunter® Receptor Tyrosine Kinase Internalization Assays

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2594.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $288.00
93-0563R16A AssayComplete™ Cell Plating 16 Reagent 100 mL $288.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $236.00
92-0029 AssayComplete™ Hygromycin B 20 mL $175.00
92-0030 AssayComplete™ G418 20 mL $133.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $92.00