PathHunter® U2OS IL18R1/IL18RAP Dimerization Cell Line

Please refer to the new clone for the assay: 93-1131C3 (PathHunter® U2OS IL18R1/IL18RAP Dimerization Cell Line)

The PathHunter® Dimerization assay detects ligand induced dimerization of two subunits of a receptor-dimer pair. The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA). Cytoplasmic tail may have been deleted from one or both receptors. Binding of an agonist to one receptor subunit induces it to interact with its dimer partner, forcing complementation of the two enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-1044C3 2 vials

Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

Related Family Members:

Receptor Family
Interleukin Receptor
Coupling
N/A
Accession Number
NM_003855.3
Receptor Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
IL-18
  • [User Manual] PathHunter® Dimerization Assay

    PathHunter® Dimerization Assays provide a robust, highly sensitive and easy-to-use cell-based functional assay to study various protein activities in a cell. The assays allow for the monitoring of receptor-receptor interactions at the&...

    [User Manual] PathHunter® Dimerization Assay