PathHunter® U2OS FcgRIIb Clustering Cell Line

The PathHunter FcγRIIb clustering cell line is a stable, engineered cell line designed to increase signal produced from a Checkpoint Cell Line via antibody-mediated crosslinking of the receptors. This product is a companion product used to determine cross-linking based increases in potency of agonist antibodies. The cell line included overexpresses FcγRIIb Fcg receptor. When co-cultured with a Checkpoint Cell Line in the presence of agonist antibody, the Fcg receptor binds to the antibody, inducing receptor clustering. The induced receptor clustering leads to increased activation and downstreamsignaling, leading to increased EFC signal production. This stable modified cell line is provided as two vials of cryopreserved cells.

Catalog No. Size Price Quantity
93-1133C3 2 Vials $10249.00

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Related Family Members:

Receptor Family
IgSF (Immunoglobulin Fc receptor )
Accession Number
Prolink™ Tag
Cell Background
Signaling assay cells were seeded in a 96-well plate and then pre-incubated with a control antibody, followed by coincubation with the clustering cell line using the assay conditions described below. Following stimulation, signal was detected using the PathHunter Detection Kit according to the recommended protocol. Please refer to page 2 for recommended assay reagents, detection reagents, and control compounds.
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