PathHunter translocation EA parental cell lines take advantage of DiscoverX’s proprietary enzyme fragment complementation technology. The cell lines are engineered to express a large fragment of β-galactosidase (β-gal) called enzyme acceptor (EA) fused to a reporter protein (Reporter-EA) which is localized to a specific subcellular compartment. Introduction of a smaller fragment of β-gal called ProLink™ (PK) fused to a target protein (Target-PK) into the cells allows for development of assays to monitor translocation of the Target-PK into this subcellular compartment. Assays using these cell lines can be used to screen and study ligands (e.g. agonists/antagonists, pharmacological chaperones, etc.) or potentially other experimental manipulations that results in translocation of the target protein to the compartment where the Reporter-EA resides. Co-localization of these two proteins forces complementation of the two β-gal enzyme fragments (EA and PK), which results in a functional enzyme which hydrolyzes PathHunter detection reagents to generate a chemiluminescent signal.