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PathHunter® U2OS CSF3R-JAK1 Functional Assay

PathHunter® CTK Functional cell lines are engineered to co-express a ProLink™ (PK) tagged Cytokine receptor, an untagged CTK, and an Enzyme Acceptor (EA) tagged SH2 domain. Activation of the Receptor-PK induces phosphorylation of the receptor by the CTK. This leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0809C3 2 vials $19995.00

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Related Family Members:

Receptor Family
Cytosolic Tyrosine Kinase
Coupling
N/A
Accession Number
NM_000760
Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
G-CSF (Human)
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] PathHunter® Cytosolic Tyrosine Kinase Functional Assay

    PathHunter® Cytosolic Tyrosine Kinase (CTK) Functional Assays are functional cell-based assays used for identification, screening or profiling of agonists, small molecule inhibitors and therapeutic biologics (peptides, proteins and various types of antibodies). Additionally, they have non-R&D applications as bioassays for QC testing and testing for anti...

    [User Manual] PathHunter® Cytosolic Tyrosine Kinase Functional Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2518.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R16A AssayComplete™ Cell Plating 16 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $245.00
92-0029 AssayComplete™ Hygromycin B 20 mL $169.00
92-0030 AssayComplete™ G418 20 mL $129.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $89.00
92-0028 AssayComplete™ Puromycin 1 mL $69.00