Find a Product

PathHunter® U2OS CD86 Ligand Cell Line

Checkpoint Signaling assays are engineered to co-express a ProLink™ (PK) tagged immune checkpoint receptor and an Enzyme Acceptor (EA) tagged SH2 domain. Ligand engagement leads to receptor activation and phosphorylation, resulting in SH2-EA recruitment to the receptor, and forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-1093C3 2 vials $9945.00

Related Family Members:

Receptor Family
T cell receptor ligand (inhibitory)
Coupling
N/A
Accession Number
NM_175862.4
Species
Human
Prolink™ Tag
None
Cell Background
U2OS
Agonist/Ligand
Anti-CTLA4 Antibody [14D3]
Antagonist/Inhibitor
Anti-CTLA4 Antibody

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0933 PathHunter® Bioassay Detection Kit 50 mL (1,000 dp, 96-well) $4366.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $245.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $89.00
92-0028 AssayComplete™ Puromycin 1 mL $69.00