PathHunter® U2OS CD86 Ligand Cell Line

The PathHunter CD86 Ligand cell line is a stable, engineered cell line designed for use with CTLA4 Checkpoint Signaling Cell Lines. When co-cultured, the CD86 expressed in this cell line will induce activity of CTLA4 receptors, which may be assayed through SH2 domain protein recruitment. This product is a companion product designed to work with Immunoglobulin Superfamily Checkpoint Assays. The cell line included overexpresses CD86 cell surface ligand. When co-cultured with the CTLA4 Checkpoint Signaling Cell Line, the CD86 ligand binds to the checkpoint receptor, inducing receptor activity. This receptor activity leads to recruitment of the relevant SH2 domain protein to the checkpoint receptor inside the receptor cell line and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.

Catalog No. Size Price Quantity
93-1093C3 2 vials $10249.00

For account specific pricing, please contact Customer Service at
Receptor Family
IgSF (immune inhibitory receptor ligand)
Accession Number
Prolink™ Tag
Cell Background
Anti-CTLA4 Antibody [14D3]
Anti-CTLA4 Antibody [14D3]
Signaling assay cells were seeded in a 96-well plate and then pre-incubated with a control antibody, followed by coincubation with the ligand cell line using the assay conditions described below. Following stimulation, signal was detected using the PathHunter Detection Kit according to the recommended protocol. Please refer to page 2 for recommended assay reagents, detection reagents, and control compounds.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2619.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $349.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $239.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00
92-0028 AssayComplete™ Puromycin 1 mL $79.00