PathHunter® U2OS CD137 Signaling Cell Line

The PathHunter CD137 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of CD137 induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein as well as untagged CD137. Upon activation of the receptor by its ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA. This stable modified cell line is provided as two vials of cryopreserved cells.

Catalog No. Size Price Quantity
93-1089C3 2 vials $36669.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.
Receptor Family
Tumor Necrosis Factor Receptor Superfamily Receptor
Coupling
N/A
Accession Number
NM_001561.5
Species
Human
Prolink™ Tag
None
Cell Background
U2OS
Agonist/Ligand
4-1BB-L
Cells were seeded in a 96-well plate, incubated at 37°C / 5% CO2 followed by stimulation with a control ligand, as defined in the assay conditions below. After stimulation, assay signal was detected using the PathHunter® detection kit according to the recommended protocol. Please refer to page 2 for recommended assay reagents, detection reagents, and control compounds.
  • [User Manual] PathHunter® Checkpoint Assay - TNF Receptor Superfamily (TNFRSF)

    The PathHunter Checkpoint Signaling Assay for Tumor Necrosis Factor Receptor Superfamily (TNFRSF) members (or receptors) is a simple, highly specific assay to enable discovery and development of small molecule and biologic drugs targeting the TNFR superfamily of receptors that activate the non-canonical NF-kB pathway through NF-kB inducing kinase (NIK). The assay is a robust measurement of co-s...

    [User Manual] PathHunter® Checkpoint Assay - TNF Receptor Superfamily (TNFRSF)

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0812 PathHunter® PL/PK Detection kit 4,000 dp (384-well) $4289.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $349.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $239.00
92-0030 AssayComplete™ G418 20 mL $139.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00
92-0028 AssayComplete™ Puromycin 1 mL $79.00