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PathHunter® U2OS CD137 Signaling Assay

Co-stimulatory checkpoint receptor signaling assays measure activation of a member of the TNFR family of receptors, which activates the non-canonical NF-kB pathway through NF-kB inducing kinase (NIK). The cell line is engineered to express NIK labeled with enzyme donor (ED). NIK protein levels inside the cell are tightly maintained at low levels by the proteasome, and increase in response to pathway activation. Addition of exogenous Enzyme Acceptor (EA) forces complementation of the ED and EA enzyme fragments resulting in the formation of a functional β-gal enzyme that hydrolyzes substrate to generate a chemiluminescent signal. The assays may rely on either endogenous receptors or exogenously expressed target receptors.

Catalog No. Size Price Quantity
93-1089C3 2 vials $35597.00

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Related Family Members:

Receptor Family
TNFR Superfamily Receptor
Coupling
N/A
Accession Number
NM_001561.5
Species
Human
Prolink™ Tag
None
Cell Background
U2OS
Agonist/Ligand
4-1BB-L
  • [User Manual] PathHunter® Co-Stimulatory Checkpoint Assay

    The PathHunter Co-Stimulatory Checkpoint Assay is a simple, highly specific assay to enable discovery and development of small molecule and biologic drugs targeting the TNFR family of receptors that activate the non-canonical NF-kB pathway through NF-kB inducing kinase (NIK). The assay is a robust measurement of co-stimulatory checkpoint receptors using cells...

    [User Manual] PathHunter® Co-Stimulatory Checkpoint Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0812 PathHunter® PL/PK Detection kit 4,000 dp (384-well) $4140.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $245.00
92-0030 AssayComplete™ G418 20 mL $129.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $89.00
92-0028 AssayComplete™ Puromycin 1 mL $69.00