PathHunter® U2OS c-Met Functional Assay

PathHunter® RTK Functional cell lines are engineered to co-express a ProLink™ (PK) tagged RTK, an Enzyme Acceptor (EA) tagged SH2 domain, and, in some cases, an untagged co-receptor. Activation of the RTK-PK induces receptor dimerization, leading to SH2-EA recruitment, and forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0632C3 2 vials

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Related Family Members:

Receptor Family
Receptor Tyrosine Kinase
Coupling
NA
Accession Number
NM_000245.2
Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
HGF
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist for 180 minutes at room temperature. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2619.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $349.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R16A AssayComplete™ Cell Plating 16 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $239.00
92-0029 AssayComplete™ Hygromycin B 20 mL $179.00
92-0030 AssayComplete™ G418 20 mL $139.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00