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KILR® THP-1 Cell Pool

KILR cell lines are engineered to express an enhanced Prolabel (ePL) tagged housekeeping gene and may sometimes overexpress an untagged version of a receptor. Once the cells have been lysed the ePL-tagged protein is released into the media. Addition of enzyme acceptor (EA) will cause the complementation of the β-galactosidase enzyme fragments, EA and ePL. The resulting functional enzyme will hydrolyze its substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
97-1011P014 Cell Pool $13514.00

Cell Background
THP-1
Cell Type
Monocyte (AML)
Culture Mode
Suspension
Agonist/Ligand
Digitoxin
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2 for the indicated amount of time. Antibody was added and opsonized for the indicated time (below). Effector cells were added and the plate was incubated at 37°C/5% CO2 using the assay conditions described below. Target cell death was detected using the KILR detection reagent according to the recommended protocol. Additional reagents needed are noted on this document.
  • [User Manual] KILR® Assay for Suspension Cell Lines

    The KILR cytotoxicity cell line enables the development of a simple, non-radioactive and dye-free method to specifically measure target cell death in a co-culture. The KILR assay has several applications in iOnc drug development including measuring target cell death through Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity ...

    [User Manual] KILR® Assay for Suspension Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
97-0001M KILR® Detection Kit 1,000 dp (96-well) $1263.00
92-3101G AssayComplete™ Cell Culture Kit-101 Kit $339.00
92-5105FR AssayComplete™ Freezing Reagent F5 2 x 50 mL $299.00
93-0563R39A AssayComplete™ Cell Plating 39 Reagent 100 mL $299.00
92-4106TR AssayComplete™ Thawing Reagent T6 100 mL $245.00
92-0030 AssayComplete™ G418 20 mL $129.00