ChemiScreen™ MC5 Melanocortin Receptor Stable Cell Line

The melanocortin system consists of five seven-transmembrane spanning G-protein coupled receptors, MC1-MC5 (Gantz and Fong, 2003). Among the five members of the melanocortin receptor family, MC2 and MC5 are expressed in peripheral tissues. The MC2 receptor (ACTH receptor) is almost exclusively expressed in the adrenal cortex whereas MC5 is expressed in several organs including the adrenal cortex. Studies have shown that targeted disruption of the MC5R gene produced mice with a severe defect in water repulsion and thermoregulation caused by decreased production of sebaceous lipids (Chen et al., 1997). In addition, MC5 is required for secretion from several exocrine glands, indicating that melanocortin peptides integrate the function of exocrine glands (Entwistle et al., 1990). Cloned human MC5 -expressing cell line is made in the Chem-10 host, which supports high levels of recombinant MC5 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MC5.

Catalog No. Size Price Quantity
HTS155C 2 Vials $18,779.00

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Application
Calcium Flux Assay, cAMP Accumulation
Family
Melanocortin
Target
MC5
Accession Number
NM_005913
Species
Human
Host Cell Type
Chem-10
Content
2 Vials of mycoplasma-free cells, 1 ml per vial
Storage
Cell vials are to be stored in the vapor phase of liquid N2.
  • [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines

    The ChemiSCREEN and ChemiBRITE Cell Lines are designed to enable the measurement of intracellular calcium levels when a GPCR of interest has been activated by a ligand. These cell lines are suitable for studying small molecules as well as biologics. The assay protocol has been optimized for use in either a 384-well or 96-well format.

    ChemiSCREEN and ChemiBRITE cells stably expres...

    [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines