ChemiBrite™ A2A Adenosine Receptor Stable Cell Line

Extracellular adenosine mediates a multitude of biological effects, including wakefulness, antiarrythmia, bronchoconstriction and response to ischemia and oxidative stress. A family of four G protein-coupled adenosine receptors, A1, α2A, α2B and A3, is responsible for these effects. α2A, which couples to Gs, is expressed in basal ganglia and immune cells. α2A reduces ischemia-induced inflammation; however, α2A antagonists protect from neurodegeneration during Parkinson's disease (Chen, 2003). Caffeine, the most widely used psychoactive drug, is a nonselective adenosine receptor antagonist, but its psychomotor stimulant affect is attributed to α2A expressed on striatal projection neurons (Fisone et al., 2004). Cloned α2A receptor-expressing ChemiBrite cells were made by stable transfection of HEK293 cells with ChemiBrite clytin and the receptor and a promiscuous G protein to couple the receptor to the calcium signaling pathway. These stability-tested cells are ready for luminescent analysis of agonists, antagonists and modulators at the α2A receptor.

Catalog No. Size Price Quantity
HTS048L 2 Vials $9,389.00

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Application
Calcium Flux Assay, cAMP Accumulation
Family
Adenosine
Target
A2A
Accession Number
BC017830
Species
Human
Host Cell Type
HEK 293
Content
2 Vials of mycoplasma-free cells, 1 ml per vial
Storage
Cell vials are to be stored in the vapor phase of liquid N2.
  • [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines

    The ChemiSCREEN and ChemiBRITE Cell Lines are designed to enable the measurement of intracellular calcium levels when a GPCR of interest has been activated by a ligand. These cell lines are suitable for studying small molecules as well as biologics. The assay protocol has been optimized for use in either a 384-well or 96-well format.

    ChemiSCREEN and ChemiBRITE cells stably expres...

    [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines