ChemiScreen™ MCH2 Melanin-concentrating Hormone Receptor Stable Cell Line

Melanin-concentrating hormone (MCH) is a cyclic, 19 amino acid peptide produced in the lateral hypothalamus and other regions of the mammalian brain (Bittencourt et al., 1992). When administered intracerebrally or overexpressed transgenically in rodents, MCH stimulates food intake and weight gain (Ludwig et al., 2001). Two G protein-coupled receptors for MCH, MCHR1 and MCHR2, have been identified in humans, although rodents appear only to have MCHR1 (Tan et al., 2002). Both MCHR1 and MCHR2 are expressed primarily in brain (An et al., 2001). MCHR1 has been demonstrated by genetic and pharmacological methods in rodent models to mediate obesity caused by a high fat diet or leptin deficiency (Borowsky et al., 2002; Segal-Lieberman, et al., 2003; Marsh et al., 2002). However, MCHR2 might play a similar role in the species that express it. The cloned human MCHR2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MCHR2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between MCH and MCHR2.

Catalog No. Size Price Quantity
HTS026C 2 Vials $18,779.00

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Calcium Flux Assay
Melanin-Concentrating Hormone
Accession Number
Host Cell Type
2 Vials of mycoplasma-free cells, 1 ml per vial
Cell vials are to be stored in the vapor phase of liquid N2.
  • [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines

    The ChemiSCREEN and ChemiBRITE Cell Lines are designed to enable the measurement of intracellular calcium levels when a GPCR of interest has been activated by a ligand. These cell lines are suitable for studying small molecules as well as biologics. The assay protocol has been optimized for use in either a 384-well or 96-well format.

    ChemiSCREEN and ChemiBRITE cells stably expres...

    [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines