ChemiScreen™ CCR2B Receptor Stable Cell Line

CCR2 is a GPCR that is expressed on monocytes, dendritic cells, natural killer cells, basophils and neutrophils, and binds to MCP-1, 3 and 4, members of the MCP (monocyte chemoattractant protein) family of chemokines (Olson and Ley, 2002). Alternative splicing of the CCR2 gene results in two variants, CCR2A and CCR2B, that differ in their C-terminal tails (Wong and Charo, 1997). CCR2 plays a protective role in some conditions: mice lacking the gene encoding CCR2 develop age-related macular degeneration (Ambati et al., 2003), and in experimental models of rheumatoid arthritis, Ccr2-null mice develop a phenotype of greater severity than wild-type mice (Quinones et al., 2004). However, CCR2 appears to promote the pathogenesis of graft-versus-host disease and pulmonary and kidney fibrosis (Kitagawa et al., 2004; Rao et al., 2003). The cloned human CCR2B-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR2B expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of CCR2B.

Catalog No. Size Price Quantity
HTS007C 2 Vials $18,779.00

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Application
Calcium Flux Assay
Family
Chemokine
Target
CCR2
Accession Number
U03905
Species
Human
Host Cell Type
Chem-1
Content
2 Vials of mycoplasma-free cells, 1 ml per vial
Storage
Cell vials are to be stored in the vapor phase of liquid N2.
  • [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines

    The ChemiSCREEN and ChemiBRITE Cell Lines are designed to enable the measurement of intracellular calcium levels when a GPCR of interest has been activated by a ligand. These cell lines are suitable for studying small molecules as well as biologics. The assay protocol has been optimized for use in either a 384-well or 96-well format.

    ChemiSCREEN and ChemiBRITE cells stably expres...

    [User Manual] ChemiSCREEN™ and ChemiBRITE™ Cell Lines