PrecisION® hGABAA α5/β3/γ2 Recombinant Stable Cell Line

Gamma-aminobutyric acid (GABA)-gated ion channels are widely distributed in the mammalian brain and are major mediators of inhibitory synaptic transmission. A typical GABAA ion channel has a pentameric structure consisting of 5 protein subunits, often α, β and γ, combining to form a central ion conducting pore across the cell membrane. In humans there are six genes that encode α subunits, three that encode β, three that encode γ, and an additional seven genes that encode other subunits whose function is less-well understood than the α, β and γ subunits. GABAA ion channels open and close in response to secretion of GABA from presynaptic terminals. GABAA α5 channels are typically comprised of α5, β3, and γ2 subunits and this combination is preferentially expressed in the mammalian hippocampus (Wisden et al., 1992). Much pharmaceutical research interest has focused on α5-selective “inverse agonists” at the benzodiazepine (BZ) site, which decrease the current elicited by a concentration of GABA that produces a 20% response, i.e. an EC20 (Atack et al. 2006, Dawson et al. 2006). These compounds show promise as dementia treatments because in animal models they enhance cognition with minimal proconvulsant or anxiogenic side-effects.

Catalog No. Size Price Quantity
CYL3073 2 Vials Inquire

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Application
Electrophysiology assay (Conventional and automated patch clamp platforms)
Family
Chloride, Ligand-Gated
Target
GABAA α5/β3/γ2
Accession Number
NM_000810; NM_000814; NM_000816
Species
Human
Host Cell Type
HEK 293
Content
2 Vials of mycoplasma-free cells, 1 mL per vial aliquots containing 2M to 4M cells/mL in DMSO
Storage
Vials are to be stored in vapor phase of liquid N2