PathHunter® OXER1 Total GPCR Internalization U2OS Cell Line

PathHunter® Total GPCR Internalization cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR, and an Enzyme Acceptor (EA) tag localized to the plasma membrane. Membrane bound, PK-tagged receptors are in close proximity to the EA, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a high basal chemiluminescent signal. Activation of the GPCR-PK induces internalization of the receptor, which dissociates the EA/PK complementation, and results in a loss of chemiluminescent signal.

Catalog No. Size Price Quantity
93-0955C3 2 vials $21,783.00

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Related Family Members:

Receptor Family
Leukotriene
Coupling
Gi/Go
Accession Number
NM_148962.4
Receptor Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
5-OxoETE
Cells were plated in a 384-well plate and incubated for atleast 1 hour at 37°C/5% CO2 to allow the cells to attach. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,857.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $379.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $309.00
93-0563R5A AssayComplete™ Cell Plating 5 Reagent 100 mL $309.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $249.00
92-0029 AssayComplete™ Hygromycin B 20 mL $199.00
92-0030 AssayComplete™ G418 20 mL $199.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $109.00