PathHunter® NCI-H460 CDC25A Degradation Cell Line

The PathHunter® assay monitors the interaction of two proteins in a whole cell, homogeneous assay format using enzyme fragment complementation. In this system, a small 42 AA enzyme fragment, ProLabel is appended to the protein of interest. The larger enzyme fragment EA (Enzyme Acceptor) resides in the kit. Activation of the pathway results in protein degradation, which can be detected using the PathHunter® ProLabel and ProLink Detection Kit (93-0812). The signal can be read on any standard luminometer.

Catalog No. Size Price Quantity
93-0776C12 2 vials $20,599.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.
Receptor Family
Cell Cycle Protein
Accession Number
NM_001789.2
Species
Human
Prolink™ Tag
PK2
Cell Background
NCI-H460
Agonist/Ligand
Doxorubicin Hydrochloride
PathHunter Cells were plated in a 384-well plate at 5000 cells/well and stimulated with the known ligand for 30 minutes. Signal was detected using the PathHunter® ProLabel and ProLink Detection Kit (93-0812) according to the recommended protocol.
  • [User Manual] PathHunter® Pathway Assay

    PathHunter® Pathway Assays, when used in conjunction with the respective detection kit (indicated in the target specific data sheet) provide a cell-based functional assay to study various protein activities in a cell. The assays described in this booklet have been validated for use in 384-well microplate formats.

    [User Manual] PathHunter® Pathway Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0812 PathHunter® PL/PK Detection kit 4,000 dp (384-well) $4,289.00
92-3101G AssayComplete™ Cell Culture Kit-101 Kit $349.00
92-5105FR AssayComplete™ Freezing Reagent F5 2 x 50 mL $299.00
93-0563R25A AssayComplete™ Cell Plating 25 Reagent 100 mL $299.00
92-4106TR AssayComplete™ Thawing Reagent T6 100 mL $239.00
92-0030 AssayComplete™ G418 20 mL $139.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00