PathHunter® MTNR1A Total GPCR Internalization DLD1 Cell Line

PathHunter® Total GPCR Internalization cell lines are engineered to co-express the Enzyme Acceptor (EA) tagged GPCR, and a ProLink™ (PK) tag localized to the endosomes. Activation of the GPCR-EA induces internalization of the receptor in PK-tagged endosomes, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0847C13 2 vials $20,799.00

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Related Family Members:

Receptor Family
Melatonin
Coupling
Gi/Go
Accession Number
NM_005958
Receptor Species
Human
Prolink™ Tag
EA
Cell Background
DLD1
Agonist/Ligand
2-Iodomelatonin
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,649.00
92-3101G AssayComplete™ Cell Culture Kit-101 Kit $359.00
92-5105FR AssayComplete™ Freezing Reagent F5 2 x 50 mL $299.00
93-0563R25A AssayComplete™ Cell Plating 25 Reagent 100 mL $299.00
92-4106TR AssayComplete™ Thawing Reagent T6 100 mL $239.00
92-0029 AssayComplete™ Hygromycin B 20 mL $189.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00
92-0028 AssayComplete™ Puromycin 1 mL $89.00