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KILR® MDA-MB-231 Cell Pool

KILR cell lines are engineered to express an enhanced Prolabel (ePL) tagged housekeeping gene and may sometimes overexpress an untagged version of a receptor. Once the cells have been lysed the ePL-tagged protein is released into the media. Addition of enzyme acceptor (EA) will cause the complementation of the β-galactosidase enzyme fragments, EA and ePL. The resulting functional enzyme will hydrolyze its substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
97-1023P036 Cell Pool $13514.00

Cell Background
MDA-MB-231
Cell Type
Breast cancer
Culture Mode
Adherent
Agonist/Ligand
Triton X-100
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2 for the indicated amount of time. Antibody was added and opsonized for the indicated time (below). Effector cells were added and the plate was incubated at 37°C/5% CO2 using the assay conditions described below. Target cell death was detected using the KILR detection reagent according to the recommended protocol. Additional reagents needed are noted on this document.
  • [User Manual] KILR® Cytotoxicity Assay−Adherent Target Cell Lines

    The KILR cytotoxicity cell line enables the development of a simple, non-radioactive and dye-free method to specifically measure target cell death in a co-culture. The KILR assay has several applications in Cancer Immunotherapy (iOnc) drug development including measuring target cell death through Antibody-Dependent Cell-mediated Cytotoxicity, ADCC, Complement-D...

    [User Manual] KILR® Cytotoxicity Assay−Adherent Target Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
97-0001M KILR® Detection Kit 1,000 dp (96-well) $1263.00
92-3105G AssayComplete™ Cell Culture Kit-105 Kit $339.00
92-5101FR AssayComplete™ Freezing Reagent F1 2 x 50 mL $299.00
93-0563R39A AssayComplete™ Cell Plating 39 Reagent 100 mL $299.00
92-4101TR AssayComplete™ Thawing Reagent T1 100 mL $245.00
92-0030 AssayComplete™ G418 20 mL $129.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $89.00