PathHunter® Jurkat mPD-1 Signaling Cell Line

The PathHunter Checkpoint Signaling Assay consists of human cells engineered to stably express an ED-tagged mouse immune checkpoint receptor, while EA is fused to either the full length or the phosphotyrosine-binding SH2 domain of an intracellular signaling protein such as Grb2 or SHP1. Engagement of the receptor by its ligand, or agonist antibodies results in phosphorylation of the receptor’s cytosolic tail. The SH2-domain fused to EA binds the phosphorylated receptor, forcing complementation of ED and EA, resulting in formation of an active β-gal enzyme, which hydrolyzes the substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-1158C19 2 vials $36669.00

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Related Family Members:

Receptor Family
IgSF (immune inhibitory receptor)
Accession Number
Prolink™ Tag
Cell Background
Anti-mPD-L1 Antibody
Anti-mPD-L1 Antibody
Signaling assay cells were seeded in a 96-well plate and then pre-incubated with a control antibody, followed by coincubation with the ligand cell line using the assay conditions described below. Following stimulation, signal was detected using the PathHunter Detection Kit according to the recommended protocol. Please refer to page 2 for recommended assay reagents, detection reagents, and control compounds.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0933 PathHunter® Bioassay Detection Kit 75 mL (1,000 dp, 96-well) $4659.00
92-3101G AssayComplete™ Cell Culture Kit-101 Kit $349.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00
92-5105FR AssayComplete™ Freezing Reagent F5 2 x 50 mL $299.00
92-4106TR AssayComplete™ Thawing Reagent T6 100 mL $239.00
92-0029 AssayComplete™ Hygromycin B 20 mL $179.00
92-0030 AssayComplete™ G418 20 mL $139.00