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PathHunter® Jurkat CTLA4 Signaling Assay

The PathHunter Checkpoint Signaling Assay consists of human cells engineered to stably express an ED-tagged immune checkpoint receptor, while EA is fused to the phosphotyrosine-binding SH2 domain of an intracellular signaling protein such as Grb2 or SHP1. Engagement of the receptor by its ligand, or agonist antibodies results in phosphorylation of the receptor’s cytosolic tail. The SH2-domain fused to EA binds the phosphorylated receptor, forcing complementation of ED and EA, resulting in formation of an active β-gal enzyme, which hydrolyzes the substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-1094C19 2 vials $35597.00

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Related Family Members:

Receptor Family
T cell receptor (inhibitory)
Coupling
N/A
Accession Number
NM_005214.4
Species
Human
Prolink™ Tag
PK1
Cell Background
Jurkat
Antagonist/Inhibitor
Anti-CTLA4 Antibody [14D3]

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0933 PathHunter® Bioassay Detection Kit 50 mL (1,000 dp, 96-well) $4497.00
92-3101G AssayComplete™ Cell Culture Kit-101 Kit $329.00
92-5105FR AssayComplete™ Freezing Reagent F5 2 x 50 mL $279.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $279.00
92-4106TR AssayComplete™ Thawing Reagent T6 100 mL $229.00
92-0029 AssayComplete™ Hygromycin B 20 mL $169.00
92-0030 AssayComplete™ G418 20 mL $129.00