PathHunter® Jurkat CTLA4 Signaling Assay
Checkpoint Signaling assays are engineered to co-express a ProLink™ (PK) tagged immune checkpoint receptor and an
Enzyme Acceptor (EA) tagged SH2 domain. Ligand engagement leads to receptor activation and phosphorylation,
resulting in SH2-EA recruitment to the receptor, and forcing complementation of the two β-galactosidase enzyme
fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.
- Cytotoxic T-lymphocyte protein 4
- Receptor Family
- T cell receptor (inhibitory)
- Accession Number
- Prolink™ Tag
- Cell Background
- Available as a Service?
- Anti-CTLA4 Antibody [14D3]
The PathHunter Checkpoint Receptor Signaling Assay is a simple, highly specific assay to enable discovery and development of small molecule and biologic drugs targeting immune checkpoint receptors.