PathHunter® Jurkat BTLA Signaling Cell Line

The PathHunter BTLA Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. HVEM) based activation of BTLA receptors via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged BTLA target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.

Catalog No. Size Price Quantity
93-1112C19 2 vials $36669.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Receptor Family
IgSF (immune inhibitory receptor)
Coupling
N/A
Accession Number
NM_181780.3
Species
Human
Prolink™ Tag
PK1
Cell Background
Jurkat
Antagonist/Inhibitor
Anti-BTLA Antibody [6F4]
Signaling assay cells were seeded in a 96-well plate and then pre-incubated with a control antibody, followed by coincubation with the ligand cell line using the assay conditions described below. Following stimulation, signal was detected using the PathHunter Detection Kit according to the recommended protocol. Please refer to page 2 for recommended assay reagents, detection reagents, and control compounds.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0933 PathHunter® Bioassay Detection Kit 75 mL (1,000 dp, 96-well) $4659.00
92-3101G AssayComplete™ Cell Culture Kit-101 Kit $349.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00
92-5105FR AssayComplete™ Freezing Reagent F5 2 x 50 mL $299.00
92-4106TR AssayComplete™ Thawing Reagent T6 100 mL $239.00
92-0029 AssayComplete™ Hygromycin B 20 mL $179.00
92-0030 AssayComplete™ G418 20 mL $139.00