PathHunter® HTR2A Total GPCR Internalization U2OS Cell Line

This product has been discontinued. Please contact Technical Support for more information regarding custom development or alternate products.

PathHunter® Total GPCR Internalization cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR, and an Enzyme Acceptor (EA) tag localized to the endosomes. Activation of the GPCR-PK induces internalization of the receptor in EA-tagged endosomes, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0807C3 2 vials

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Related Family Members:

Receptor Family
5-Hydroxytryptamine
Coupling
Gq
Accession Number
NM_000621.3
Receptor Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
Serotonin/5-HT
  • [User Manual] PathHunter® Total GPCR Internalization Assay

    PathHunter® Total GPCR Internalization Assays are non-imaging, non-antibody based chemiluminescent detection assays that provide a direct and quantitative measurement of internalized GPCRs localized in early endosomes. This allows the internalization of GPCRs to be quantitatively measured in live cells with-out the need for expensive microscopy. The PathHunter system combines eng...

    [User Manual] PathHunter® Total GPCR Internalization Assay