PathHunter® HEK 293 IKZF3v1 Degradation Cell Line

PathHunter Degradation cell lines are engineered to express an Enzyme Donor (ED) tagged target protein. Activation of the pathway results in degradation of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer, lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-1165C1 2 vials $21,783.00

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Receptor Family
Ikaros family of ZFN transcription factors
Coupling
N/A
Accession Number
NM_012481.5
Receptor Species
Human
Prolink™ Tag
ePL
Cell Background
HEK 293
Agonist/Ligand
Lenalidomide
PathHunter Cells were plated in a 384-well plate at 5000 cells/well and stimulated with the known ligand for 30 minutes. Signal was detected using the PathHunter® ProLabel and ProLink Detection Kit (93-0812) according to the recommended protocol.
  • [User Manual] PathHunter® Pathway Assay

    PathHunter® Pathway Assays, when used in conjunction with the respective detection kit (indicated in the target specific data sheet) provide a cell-based functional assay to study various protein activities in a cell. The assays described in this booklet have been validated for use in 384-well microplate formats.

    [User Manual] PathHunter® Pathway Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0812 PathHunter® PL/PK Detection kit 4,000 dp (384-well) $4,779.00
92-3105G AssayComplete™ Cell Culture Kit-105 Kit $379.00
92-5101FR AssayComplete™ Freezing Reagent F1 2 x 50 mL $329.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $329.00
92-4101TR AssayComplete™ Thawing Reagent T1 100 mL $249.00
92-0030 AssayComplete™ G418 20 mL $227.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $109.00