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PathHunter® HEK 293 EDG3 β-Arrestin Cell Line

PathHunter® β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0217C1 2 vials $22,219.00
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Related Family Members:

Receptor Family
Lysophospholipid (S1P)
Coupling
Gq
Accession Number
NM_005226
Receptor Species
Human
β-Arrestin isoform
β-Arrestin-2
Prolink™ Tag
PK1
Cell Background
HEK 293
Agonist/Ligand
Sphingosine 1-phosphate
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.

  • [User Manual] PathHunter® β-Arrestin Assay for GPCR Cell Lines

    PathHunter β-Arrestin cell lines are stable clonal cell lines that expedite drug discovery and development by providing robust response to over 90% of all known G-protein coupled receptor (GPCRs), with accurate pharmacology. This assay measures an essential pathway in GPCR activation, i.e. β-arrestin recruitment to activated GPCRs, enabling scientists...

    [User Manual] PathHunter® β-Arrestin Assay for GPCR Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,857.00
92-3105G AssayComplete™ Cell Culture Kit-105 Kit $389.00
92-5101FR AssayComplete™ Freezing Reagent F1 2 x 50 mL $334.00
93-0563R7A AssayComplete™ Cell Plating 7 Reagent 100 mL $334.00
92-4101TR AssayComplete™ Thawing Reagent T1 100 mL $249.00
92-0029 AssayComplete™ Hygromycin B 20 mL $227.00
92-0030 AssayComplete™ G418 20 mL $227.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $114.00