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KILR® HCT-116 Cell Pool

KILR cell lines are engineered to express an enhanced Prolabel (ePL) tagged housekeeping gene and may sometimes overexpress an untagged version of a receptor. Once the cells have been lysed the ePL-tagged protein is released into the media. Addition of enzyme acceptor (EA) will cause the complementation of the β-galactosidase enzyme fragments, EA and ePL. The resulting functional enzyme will hydrolyze its substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
97-1040P047 2 Vials $13920.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.
Species
Human
Prolink™ Tag
ePL
Cell Background
HCT-116
Cell Type
Colorectal carcinoma
Culture Mode
Adherent
Agonist/Ligand
Triton X-100
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2 for the indicated amount of time. Antibody was added and opsonized for the indicated time (below). Effector cells were added and the plate was incubated at 37°C/5% CO2 using the assay conditions described below. Target cell death was detected using the KILR detection reagent according to the recommended protocol. Additional reagents needed are noted on this document.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
97-0001M KILR® Detection Kit 1,000 dp (96-well) $1341.00
92-3102G AssayComplete™ Cell Culture Kit-102 Kit $339.00
92-5106FR AssayComplete™ Freezing Reagent F6 2 x 50 mL $288.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $288.00
92-4108TR AssayComplete™ Thawing Reagent T8 100 mL $236.00
92-0030 AssayComplete™ G418 20 mL $133.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $92.00