Products / Cell Line / 97-1019P033
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KILR® EL4 Cell Pool

The KILR EL4 Cell Pool is a stable, engineered cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatmnets (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP).This product uses EFC technology. The cell pool is a heterogeneous population of EL4 cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces and EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells.

Catalog No. Size Price Quantity
97-1019P033 2 vials $14,779.00
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Cell Background
EL4
Cell Type
T lymphocyte
Culture Mode
Suspension
Agonist/Ligand
Triton X-100
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2 for the indicated amount of time. Antibody was added and opsonized for the indicated time (below). Effector cells were added and the plate was incubated at 37°C/5% CO2 using the assay conditions described below. Target cell death was detected using the KILR detection reagent according to the recommended protocol. Additional reagents needed are noted on this document.

  • [User Manual] KILR® Assay for Suspension Cell Lines

    The KILR cytotoxicity cell line enables the development of a simple, non-radioactive and dye-free method to specifically measure target cell death in a co-culture. The KILR assay has several applications in iOnc drug development including measuring target cell death through Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity ...

    [User Manual] KILR® Assay for Suspension Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
97-0001M KILR® Detection Kit 1,000 dp (96-well) $1,479.00
92-3101G AssayComplete™ Cell Culture Kit-101 Kit $389.00
92-5105FR AssayComplete™ Freezing Reagent F5 2 x 50 mL $334.00
93-0563R39A AssayComplete™ Cell Plating 39 Reagent 100 mL $334.00
92-4106TR AssayComplete™ Thawing Reagent T6 100 mL $249.00
92-0030 AssayComplete™ G418 20 mL $227.00