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PathHunter® EDG3 Activated GPCR Internalization U2OS Cell Line

PathHunter® Activated GPCR Internalization cell lines are engineered to co-express an untagged GPCR, Enzyme Acceptor (EA) tagged β-Arrestin, and a ProLink™ (PK) tag localized to the endosomes. Activation of the GPCR induces β-Arrestin recruitment, followed by internalization of the Receptor/Arrestin-EA complex in PK-tagged endosomes. This forces complementation of the two β-galactosidase enzyme fragments (EA and PK), forming functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0663C3 2 vials $22,219.00
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Related Family Members:

Receptor Family
Lysophospholipid (S1P)
Coupling
Gq
Accession Number
NM_005226
Receptor Species
Human
β-Arrestin isoform
β-Arrestin-2
Prolink™ Tag
None
Cell Background
U2OS
Agonist/Ligand
Sphingosine 1-phosphate
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.

  • [User Manual] PathHunter® Activated GPCR Internalization Assay

    PathHunter® Activated GPCR Internalization Assays are non-imaging, non-antibody based chemiluminescent detection assays that provide a direct and quantitative measurement of internalized GPCRs localized to early endosomes. This allows the internalization of GPCRs to be quantitatively measured in live cells without the need for expensive microscop...

    [User Manual] PathHunter® Activated GPCR Internalization Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,857.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $389.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $334.00
93-0563R26A AssayComplete™ Cell Plating 26 Reagent 100 mL $334.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $249.00
92-0029 AssayComplete™ Hygromycin B 20 mL $227.00
92-0030 AssayComplete™ G418 20 mL $227.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $114.00
92-0028 AssayComplete™ Puromycin 1 mL $97.00