PathHunter® CMKLR1 Activated GPCR Internalization U2OS Cell Line

PathHunter® Activated GPCR Internalization cell lines are engineered to co-express an untagged GPCR, Enzyme Acceptor (EA) tagged β-Arrestin, and a ProLink™ (PK) tag localized to the endosomes. Activation of the GPCR induces β-Arrestin recruitment, followed by internalization of the Receptor/Arrestin-EA complex in PK-tagged endosomes. This forces complementation of the two β-galactosidase enzyme fragments (EA and PK), forming functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0720C3 2 vials $20595.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Receptor Family
Chemerin
Coupling
Gi/Go
Accession Number
NM_004072.2
Species
Human
β-Arrestin isoform
β-Arrestin-2
Prolink™ Tag
None
Cell Background
U2OS
Agonist/Ligand
Recombinant Human Chemerin
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2594.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $288.00
93-0563R22A AssayComplete™ Cell Plating 22 Reagent 100 mL $288.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $236.00
92-0029 AssayComplete™ Hygromycin B 20 mL $175.00
92-0030 AssayComplete™ G418 20 mL $133.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $92.00
92-0028 AssayComplete™ Puromycin 1 mL $72.00