PathHunter® CHO-K1 NPR1 Functional Assay

PathHunter® Degradation cell lines are engineered to express an Enzyme Donor (ED) tagged target protein. Activation of the pathway results in degradation of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0804C2 2 vials $20595.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.
Receptor Family
Guanylate Cyclase A
Accession Number
NM_000906.3
Species
Human
Prolink™ Tag
None
Cell Background
CHO-K1
Agonist/Ligand
Human/Porcine ANP(1-28)
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control compound. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] PathHunter® Pathway Assay

    PathHunter® Pathway Assays, when used in conjunction with the respective detection kit (indicated in the target specific data sheet) provide a cell-based functional assay to study various protein activities in a cell. The assays described in this booklet have been validated for use in 384-well microplate formats.

    [User Manual] PathHunter® Pathway Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
90-0039 HitHunter® cGMP Assay 800 dp (384-well) $601.00
92-3107G AssayComplete™ Cell Culture Kit-107 Kit $339.00
92-5102FR AssayComplete™ Freezing Reagent F2 2 x 50 mL $288.00
93-0563R43A AssayComplete™ Cell Plating 43 Reagent 100 mL $288.00
92-4102TR AssayComplete™ Thawing Reagent T2 100 mL $236.00
92-0030 AssayComplete™ G418 20 mL $133.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $92.00