PathHunter® CHO-K1 GPR17 β-Arrestin Cell Line

PathHunter® β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two enzyme fragments. The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. PathHunter cell lines expressing Gq-coupled receptors can also be used to detect calcium mobilization, using the Calcium No WashPlus Detection Kit.

Catalog No. Size Price Quantity
93-0633C2 2 vials

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Related Family Members:

Receptor Family
Class A Orphan
Coupling
Unknown
Accession Number
NM_005291
Species
Human
β-Arrestin isoform
β-Arrestin-2
Prolink™ Tag
PK1
Cell Background
CHO-K1
Agonist/Ligand
MDL 29,951
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist using the assay conditions described below. Following stimulation, signal from Arrestin recruitment was detected using the PathHunter® detection kit according to the recommended protocol. Calcium mobilization was detected using the Calcium No WashPLUS detection kit. Calcium data is background subtracted and represented as ΔRFU(Relative Fluorescence units).

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2619.00
92-3107G AssayComplete™ Cell Culture Kit-107 Kit $349.00
92-5102FR AssayComplete™ Freezing Reagent F2 2 x 50 mL $299.00
93-0563R2A AssayComplete™ Cell Plating 2 Reagent 100 mL $299.00
92-4102TR AssayComplete™ Thawing Reagent T2 100 mL $239.00
92-0029 AssayComplete™ Hygromycin B 20 mL $179.00
92-0030 AssayComplete™ G418 20 mL $139.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00