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PathHunter® CHO-K1 ERα Protein Interaction Cell Line

The PathHunter® NHR Protein Interaction assays monitor the interaction of activated NHRs with Steroid Receptor Co-activator Peptides (SRCPs) using Enzyme Fragment Complementation. The cells are engineered to express two complementary fragments of β-galactosidase: the smaller ProLink™ (PK) fragment is fused to the NHR target of interest, and the larger Enzyme Acceptor (EA) is fused to the SRCP. Activation of the NHR target induces interaction of the receptor with the SRCP, forcing complementation of PK and EA. Activity of the resulting fully formed β-galactosidase enzyme is detected using a chemiluminescent substrate.

Catalog No. Size Price Quantity
93-0453C2 2 vials $10295.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Receptor Family
NHR
Coupling
N/A
Accession Number
NM_000125
Species
Human
Prolink™ Tag
PK1
Cell Background
CHO-K1
Agonist/Ligand
β-Estradiol
Cells were plated in a 384-well plate, incubated overnight at 37°C and stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® Detection kit according to the recommended protocol.
  • [User Manual] PathHunter® NHR Assay

    Nuclear Hormone Receptor (NHR) activation is comprised of four steps: 1) ligand binding to the NHR, 2) nuclear translocation, 3) recruitment of a Steroid Receptor Coactivator Peptide (SRCP), and 4) transcriptional activation of target DNA sequence. P...

    [User Manual] PathHunter® NHR Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2594.00
92-3107G AssayComplete™ Cell Culture Kit-107 Kit $339.00
92-5102FR AssayComplete™ Freezing Reagent F2 2 x 50 mL $288.00
93-0563R13A AssayComplete™ Cell Plating 13 Reagent 100 mL $288.00
92-4102TR AssayComplete™ Thawing Reagent T2 100 mL $236.00
92-0029 AssayComplete™ Hygromycin B 20 mL $175.00
92-0030 AssayComplete™ G418 20 mL $133.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $92.00