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PathHunter® CHO-K1 EDG6 β-Arrestin Cell Line

PathHunter® β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two enzyme fragments. The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. PathHunter cell lines expressing Gq-coupled receptors can also be used to detect calcium mobilization, using the Calcium No WashPlus Detection Kit.

Catalog No. Size Price Quantity
93-0227C2 2 vials $22,219.00
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Related Family Members:

Receptor Family
Lysophospholipid (S1P)
Coupling
Gi/Go
Accession Number
AY322537
Receptor Species
Human
β-Arrestin isoform
β-Arrestin-2
Prolink™ Tag
PK1
Cell Background
CHO-K1
Agonist/Ligand
Sphingosine 1-phosphate
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist using the assay conditions described below. Following stimulation, signal from Arrestin recruitment was detected using the PathHunter® detection kit according to the recommended protocol. Calcium mobilization was detected using the Calcium No WashPLUS detection kit. Calcium data is background subtracted and represented as ΔRFU(Relative Fluorescence units).

  • [User Manual] PathHunter® β-Arrestin Assay for GPCR Cell Lines

    PathHunter β-Arrestin cell lines are stable clonal cell lines that expedite drug discovery and development by providing robust response to over 90% of all known G-protein coupled receptor (GPCRs), with accurate pharmacology. This assay measures an essential pathway in GPCR activation, i.e. β-arrestin recruitment to activated GPCRs, enabling scientists...

    [User Manual] PathHunter® β-Arrestin Assay for GPCR Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,857.00
92-3108G AssayComplete™ Cell Culture Kit-108 Kit $389.00
92-5102FR AssayComplete™ Freezing Reagent F2 2 x 50 mL $334.00
93-0563R11A AssayComplete™ Cell Plating 11 Reagent 100 mL $334.00
92-4102TR AssayComplete™ Thawing Reagent T2 100 mL $249.00
92-0029 AssayComplete™ Hygromycin B 20 mL $227.00
92-0030 AssayComplete™ G418 20 mL $227.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $114.00