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cAMP Hunter™ CHO-K1 C5R1 Gi Cell Line

cAMP Hunter™ Gi cell lines overexpress naturally Gi coupled, wildtype GPCRs and are designed to detect increases in intracellular cAMP levels in response to agonist stimulation of the receptor. These cell lines are designed to be used in conjunction with the HitHunter® cAMP Assay detection kit.

Catalog No. Size Price Quantity
95-0079C2 2 vials $10,879.00
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Related Family Members:

Receptor Family
Complement peptide
Coupling
Gi/Go
Accession Number
NM_001736
Receptor Species
Human
Prolink™ Tag
None
Cell Background
CHO-K1
Agonist/Ligand
Recombinant Human Complement C5a
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist + forskolin (Figure 1A) or with forskolin only (Figure 1B). Following stimulation, signal was detected using the HitHunter® cAMP detection kit according to the recommended protocol.

  • [User Manual] cAMP Hunter™ Gs and Gi Cell Lines

    cAMP Hunter Cell Lines are non-force-coupled Gs and Gi stable clonal cell lines that expedite drug screening by providing robust responses, with accurate pharmacology. They provide the means for the measurement of an ubiquitous second messenger (cAMP) to determine the mechanism of action of ligands. Each cell line has been characterized for its appropriate GPCR...

    [User Manual] cAMP Hunter™ Gs and Gi Cell Lines

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
90-0075SM2 HitHunter® cAMP Assay for Small Molecules 200/800 dp (96-well/384-well) $484.00
90-0075SM2 HitHunter® cAMP Assay for Small Molecules 200/800 dp (96-well/384-well) $484.00
92-3107G AssayComplete™ Cell Culture Kit-107 Kit $389.00
92-5102FR AssayComplete™ Freezing Reagent F2 2 x 50 mL $334.00
93-0563R2A AssayComplete™ Cell Plating 2 Reagent 100 mL $334.00
92-4102TR AssayComplete™ Thawing Reagent T2 100 mL $249.00
92-0030 AssayComplete™ G418 20 mL $227.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $114.00