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PathHunter® CCR2 Total GPCR Internalization U2OS Cell Line

PathHunter® Total GPCR Internalization cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR, and an Enzyme Acceptor (EA) tag localized to the endosomes. Activation of the GPCR-PK induces internalization of the receptor in EA-tagged endosomes, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0679C3 2 vials $21,783.00
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Related Family Members:

Receptor Family
Chemokine
Coupling
Gi/Go
Accession Number
NM_001123396
Receptor Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
Recombinant Human MCP-1 (CCL2)
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.

  • [User Manual] PathHunter® Total GPCR Internalization Assay

    PathHunter® Total GPCR Internalization Assays are non-imaging, non-antibody based chemiluminescent detection assays that provide a direct and quantitative measurement of internalized GPCRs localized in early endosomes. This allows the internalization of GPCRs to be quantitatively measured in live cells with-out the need for expensive microscopy. The PathHunter system combines eng...

    [User Manual] PathHunter® Total GPCR Internalization Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2,857.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $379.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $309.00
93-0563R5A AssayComplete™ Cell Plating 5 Reagent 100 mL $309.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $249.00
92-0029 AssayComplete™ Hygromycin B 20 mL $199.00
92-0030 AssayComplete™ G418 20 mL $199.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $109.00