Find a Product

PathHunter® A549 IκB Degradation Cell Line

The PathHunter® assay monitors the interaction of two proteins in a whole cell, homogeneous assay format using enzyme fragment complementation. In this system, a small 42 AA enzyme fragment, ProLabel is appended to the protein of interest. The larger enzyme fragment EA (Enzyme Acceptor) resides in the kit. Activation of the pathway results in protein degradation, which can be detected using the PathHunter® ProLabel and ProLink Detection Kit (93-0812). The signal can be read on any standard luminometer.

Catalog No. Size Price Quantity
93-0538C15 2 vials $19995.00

For account specific pricing, please contact Customer Service at info@discoverx.com.
Receptor Family
Pathway
Coupling
N/A
Accession Number
NM_020529
Species
Human
Prolink™ Tag
PL
Cell Background
A549
Agonist/Ligand
TNFα (human)
PathHunter Cells were plated in a 384-well plate at 5000 cells/well and stimulated with the known ligand for 30 minutes. Signal was detected using the PathHunter® ProLabel and ProLink Detection Kit (93-0812) according to the recommended protocol.
  • [User Manual] PathHunter® Pathway Assay

    PathHunter® Pathway Assays, when used in conjunction with the respective detection kit (indicated in the target specific data sheet) provide a cell-based functional assay to study various protein activities in a cell. The assays described in this booklet have been validated for use in 384-well microplate formats.

    [User Manual] PathHunter® Pathway Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0812 PathHunter® PL/PK Detection kit 4,000 dp (384-well) $4140.00
92-3105G AssayComplete™ Cell Culture Kit-105 Kit $339.00
92-5101FR AssayComplete™ Freezing Reagent F1 2 x 50 mL $299.00
93-0563R0A AssayComplete™ Cell Plating 0 Reagent 100 mL $299.00
92-4101TR AssayComplete™ Thawing Reagent T1 100 mL $245.00
92-0030 AssayComplete™ G418 20 mL $129.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $89.00